Br J Pharmacol. Oct;(4) doi: /j x. Experimental autoimmune encephalomyelitis (EAE) as a model for multiple. La inducción de la encefalomielitis autoinmune experimental (EAE) mediante la glicoproteína de la mielina del oligodendrocito (MOG) en ratones C57BL/6. Keywords: multiple sclerosis, encephalomyelitis, autoimmune, experimental, Latin Estudios sobre la encefalomielitis autoinmune experimental (EAE) en.
|Published (Last):||12 October 2014|
|PDF File Size:||7.81 Mb|
|ePub File Size:||14.37 Mb|
|Price:||Free* [*Free Regsitration Required]|
Becher agrees that such environmental variations are a major issue but notes that if EAE experiments more often included the necessary controls, such as tests in both actively induced and adoptive transfer models, the noise generated by animal husbandry variations would matter less see also Tips section below.
This caveat applies especially in drug testing, they say. Conservar la muestra en PBS en hielo. Ver Tabla 1 para las secuencias para los conjuntos de cebadores. Baker and his colleague Sandra Amor at VU University Medical Center in the Netherlands laid out a set of guidelines that proposes minimally acceptable standards for publishing studies that use EAE as a preclinical model Baker and Amor, Their T cells are then harvested, reactivated with the immunizing antigen in culture, and injected into recipient animals.
J Mol Med Berl. For example, T-cell receptor TCR autooinmune mice carry T cells engineered to respond to specific brain antigens. A six-point scale reflects the severity of symptoms, with a score of 1 being tail paralysis, a score of 4 indicating quadriplegia, and a score of 5 defined as death. This immunisation leads to multiple small disseminated lesions of demyelination as well as micro-necroses in the brain and spinal cord and the onset of clinical symptoms. In diseased animals, clinical signs started to appear by day 8 post-induction.
Received in November, The origin and application of experimental autoimmune encephalomyelitis. In the early days of EAE, studying disease states caused by injecting brain homogenate in different species helped pinpoint how particular tissue-specific molecules produce autoimmune reactions Krishnamoorthy and Wekerle, Tips Auttoinmune sheer number of permutations of EAE models makes their use a minefield for experimental error, and diving into the literature is often not enough to develop the needed level of expertise to use EAE most effectively, Becher says.
EAE has a complex neuropharmacology, and many of the autoinumne that are in current or imminent use in MS have been developed, tested or validated on the basis of EAE studies.
EAE generally targets the spinal cord and autoinmunee the cerebellum, causing inflammation followed by demyelination and axonal damage. Recovery from symptoms can be complete or partial and the time varies with symptoms and disease severity.
In the negative control group, body weight increased steadily throughout the study, at a rate above that of diseased animals. Leptin neutralization interferes with pathogenic T cell autoreactivity in autoimmune encephalomyelitis.
A partir de entonces, resuspender el precipitado en 30 l de agua. Epub Jun This disease-progression autoinmine and other clinical features, such as the type of paralysis that occurs, depend on the animal, genetic strain, induction method, and autoantigen used Merrill, ; Miller and Karpus, ; Baxter, IL drives a pathogenic T cell population that induces autoimmune inflammation.
EAE is also the go-to model for testing the effectiveness of potential MS therapeutics. Por lo tanto, otros modelos animales tales como el modelo cuprizone se pueden utilizar para el estudio distinto de desmielinizante procesa Symptoms of the disorder persisted until the end of the study, coinciding with the chronic experumental form of EAE described by Amor et al.
Nat Rev Drug Discov. During some stages, clinical evaluation may not follow or mimic the behavior of molecular parameters, and body weight was therefore explored solely in a descriptive manner. Although sharing some features, mostly demyelination, this model, first introduced in s, encrfalomielitis from human MS in several ways.
Initially, rats, guinea pigs, and nonhuman primates comprised the largest contingent of EAE animals. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. A number of clinical and molecular variables, together with experimenral analyses, were used to demonstrate the implementation of the model.
Experimental autoimmune encephalomyelitis (EAE) as a model for multiple sclerosis (MS).
This allows the eventual development and preclinical testing of a wide range of potential therapeutic interventions. Commons category link is on Wikidata.
Success of immunomodulators in MS shifts discovery focus to neuroprotection. Experimental autoimmune encephalomyelitis EAE is the most commonly used experimental model for the human inflammatory demyelinating disease, multiple sclerosis MS.
The methodology described here enabled the implementation of an animal model that reproduces fundamental aspects of the pathogenesis of MS and is, therefore, highly useful for the study of physiopathological mechanisms, the identification of new pharmacological targets and the evaluation of specific biomolecules with therapeutic purposes.
Los pares de cebadores. Depending on the relapse-remission intervals, rats can have up to 3 bouts of disease within an experimental period.
In vitro and in vivo pharmacological models to assess demyelination and remyelination. Most researchers support experimentak utility to at least some extent, but a few dismiss it.
In the alternative approach, called adoptive transfer, animals are immunized against a particular antigen and then sacrificed.
Experimental Autoimmune Encephalomyelitis
In addition, environmental factors can be determinant in the development of EAE. It is critical, therefore, to guarantee that the researcher is well trained in the proper procedure for clinical exploration of the animal, according to the selected scale.
Para esto, homogeneizar el tejido en 1 mezcla de tiocianato de guanidinio-fenol ml, se incuba durante 10 min.
Their findings upon a transfer of inflamed patient tissue to primates was published in the Journal of Experimental Medicine.
Probar la especificidad de cada cebador. One-milliliter plastic syringes were used for the immunizations, loaded directly from the glass syringe by means of the metal capillary. In these animals, experimentap with myelin basic protein MBP or with proteolipid protein or peptides of it —also with an adjuvant—spurs the first signs of disease within 7 to 14 days.
The model for primary progressive MS is the Theiler’s virus model.
Leptin surge precedes onset of autoimmune encephalomyelitis and correlates with development of pathogenic T cell responses. The sheer number of permutations of EAE models makes their use a ejcefalomielitis for experimental error, and diving into the literature is often not enough to develop the needed level of expertise to use EAE most effectively, Becher says. In the course of his experiments, the researcher, Thomas Rivers, injected control animals with emulsified brain tissue; they developed brain-specific antibodies and a proportion of them became transiently paralyzed.